To develop UV spectrophotometric method for the estimation of Paracetamol, Domperidone and Flunarizine in the combined tablet dosage form available in the market for the treatment of migraine. The three drugs Paracetamol, Domperidone, and Flunarizine are present in the ratio of 100:2:1 which poses a problem in their simultaneous estimation. Hence, an effective and reproducible extraction method to extract out Paracetamol from the combination was developed and applied successfully to the formulation. The estimation of Paracetamol was done at 256 nm. The two drugs Domperidone and Flunarizine were simultaneously estimated by first order derivative method. Wavelengths 223.97nm (ZCP for Flunarizine) and 256.30 nm (ZCP for Domperidone) were selected for the estimation of Domperidone and Flunarizine from their overlain first order derivative spectra. The concentration of the three drugs was determined by the proposed method. The method was validated as per the ICH guidelines. Linearity was observed in concentration range of 4-12 µg/ml for Paracetamol and 10-40 µg/ml for both drugs Domperidone and Flunarizine. The accuracy of the method was evaluated by recovery studies and good recovery results were obtained between 97 % to 100 % and the relative standard deviation was found to be below 2 %. A simple, accurate, precise and economical UV-spectrophotometric method for the estimation of Paracetamol, Domperidone, and Flunarizine in combined dosage form has been developed, which can be applied for their routine analysis.
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